• Novel genes

    Novel genes

    Genes that have been predicted by Ensembl on the basis of similarity to protein or cDNA sequences.

  • Vector

    Vector

    A small DNA (ds-circular) molecule that can be used to deliver DNA into a cell.

  • Restriction enzymes

    Restriction enzymes

    Enzymes are involved in the breaking of DNA molecules at specific site.

  • DNA ligases

    DNA ligases

    Which join or recombine DNA fragments (ligation).

  • Recombinant DNA

    Recombinant DNA

    A form of artificial DNA that is created by combining two or more sequences that would not normally occur together.

  • Polymerases

    Polymerases

    Enzymes are involved in the building or synthesis of nucleic acids.

Saturday, 29 September 2012

Restriction enzymes

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Restriction enzymes are classified as endonucleases. Their biochemical activity is the hydrolysis ("digestion") of the phosphodiester backbone at specific sites in a DNA sequence. By "specific" we mean that an enzyme...

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Why is rDNA important?

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1. Better Crops (drought & heat resistance) 2. Recombinant Vaccines (ie. Hepatitis B) 3. Prevention and cure of sickle cell anemia 4. Prevention and cure of cystic fibrosis 5. Production of...

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How is Recombinant DNA made?

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There are three different methods by which Recombinant DNA is made.  1. Transformation The first step in transformation is to select a piece of DNA to be inserted into a vector. The...

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Restriction enzymes are used to make recombinant DNA

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1. Cut DNA at specific DNA sequences §             Symmetrical series of four to eight  bases §             Sticky Sticky or blunt ends...

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Recombinant DNA

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Recombinant DNA (rDNA) is a form of artificial DNA that is created by combining two or more sequences that would not normally occur together. In terms of genetic modification, it is...

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History of recombinant DNA technology

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Recombinant DNA technology is one of the recent  advances in biotechnology, which was developed by  two scientists named Boyer and Cohen in 1973. ...

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Recombinant DNA technology

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A series of procedures that are used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed from segments of two or more different DNA molecules. Under certain conditions,...

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